Streptavidin Microbeads Miltenyi Biotec

Caspase-1, Caspase-8, And Calpain Are Dispensable For IL-33 …
Incubated with streptavidin-Microbeads (Miltenyi Biotec) at 4°C for 20 min. Spleen cells were incubated with CD11c-Microbeads (Miltenyi Bio-tec) at 4°C for 20 min. F4/80 peritoneal macrophages ( 92%) and CD11c splenic DCs ( 92%) were isolated by positive selection using a … Fetch Here

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MOLECULAR BIOLOGY Immunoprecipitation Of Human B Cell Surface …
MicroBeads. After mixing, the lysate was incubated for 30 minutes with Streptavidin-peroxidase at a ratio of 1:100,000. The membrane was washed 3x with PBS. The Miltenyi Biotec GmbH Miltenyi Biotec Inc. Miltenyi Biotec Ltd.(UK) … Get Doc

Antigen-specific T Cells Labeled With Pentamer-bead
Columns (e.g. Streptavidin Microbeads, Miltenyi Biotec #130-048-102) • Streptavidin, conjugated to fluorescent label of choice • Anti-CD8 antibody, conjugated with a different fluorescent label to streptavidin • Wash buffer, de-gassed (0.1% sodium azide, 0.1% BSA in PBS) … Fetch Content

RESEARCH Matrix Protein 2 Vaccination And Protection Against …
With Streptavidin MicroBeads (Miltenyi Biotec, Auburn, CA, USA). Unlabeled T cells and labeled non–T cells were separated through the Miltenyi AutoMACS system accord-ing to the manufacturer’s instructions. Enzyme-linked Immunosorbent Spot (ELISPOT) Assay … Read More

Muscle-derived Hematopoietic Stem Cells Are Hematopoietic In …
1 10 dilution of streptavidin-conjugated microbeads (Miltenyi Biotec, Auburn, CA). Cells were then suspended to 12 103 VarioMACS magnet (Miltenyi Biotec). Cells were also fractionated by using fluorescent activated cell sorting (FACS). … Fetch This Document

IFN Inducesmonopoiesisandinhibitsneutrophildevelopmentduring …
Conjugates were visualized by streptavidin-PerCP–Cy5.5 (BD Pharmin-gen), streptavidin-PE–Cy7, or streptavidin-PE. Where possible, cells were microbeads (Miltenyi Biotec) and MACS-positive bead selection with LS columns (Miltenyi Biotec). … Document Viewer

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Donor-Specific Transplantation Tolerance: The Paradoxical …
Sciences), and positively selected with streptavidin microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) by using an autoMACS (Miltenyi Biotec). The CD4+CD25- T cells were isolated from the CD25-depleted fraction by using positive selection with anti … Access Content

Viral Double-Stranded RNA Triggers Ig Class Switching By …
Viral Double-Stranded RNA Triggers Ig Class Switching by Activating Upper Respiratory Mucosa B Cells through an Innate TLR3 Pathway Involving BAFF1 … Visit Document

Chapter 3 A Method To Isolate And Purify Human Bone Marrow …
Streptavidin microbeads, (Miltenyi Biotec; Bergisch Gladbach, Germany). 5. Streptavidin LS Columns, (Miltenyi Biotec). 6. 4- and 14-mL polypropylene Falcon tubes (Becton Dickinson Biosciences). 7. FACS Fix; 1% (v/v) formalin, 0.1 M d-glucose, 0.02% sodium azide in PBS). … Doc Viewer

Synergistic Effects Of IL-4 And IL-18 On IL-12-Dependent IFN …
Synergistic Effects of IL-4 and IL-18 on IL-12-Dependent IFN-gProduction by Dendritic Cells1 Taro Fukao, Satoshi Matsuda, and Shigeo Koyasu2 Mouse splenic dendritic cells (DCs) produce IFN-gin response to IL-12. … Visit Document